De Guzman Micko D
Graduate School, University of Perpetual Help System - Laguna, City of Biñan, Laguna, Philippines.
Acta Med Philipp. 2025 Mar 31;59(4):139-150. doi: 10.47895/amp.v59i4.8785. eCollection 2025.
Espada plant, local name for the snake plant () in the Philippines, is characterized by its upright sword-like leaves with vibrant yellow edges under the variety of Laurentii in the Asparagaceae family. This plant has been identified as a viable candidate for cancer research.
To investigate the antiproliferative and cytotoxic capabilities of a semi-purified methanolic extract of extracted as a basis for cancer research.
The plant extracts were subjected to (1) qualitative phytochemical analysis, (2) instrumentation analysis which includes Fourier Transform Infrared Spectroscopy (FTIR-ATR) and Total Flavonoid Content (TFC), to quantify bioactive ingredients, analyze structures, and evaluate biological chemicals, respectively, and tested to (3) biological assay on the HCT 116 human colorectal cancer cell line using the MTT Cytotoxic Assay.
extracts revealed the presence of flavonoids, saponins, sterols, triterpenes, alkaloids, and glycosides, all of which contain an OH group and have a high solubility in polar solvents. It correlates to the results of TFC, found to be within 266.8333 mg - 622.6801 mg presented as μg Quercetin per mL with a linear line of y=0.0005x + 0.023 with a coefficient R value of 0.9933. This finding corresponds to FTIR-ATR data, which shows a prominent broad appearance of -OH (primary and secondary alcohol) at peak 3327.21. In MTT Cytotoxic Assay, it has a minimal IC50 than Doxorubicin, as seen in Trial 2 with IC50 = 0.8012 μg/mL, while antiproliferative activity revealed that has minimal inhibitory activity in Trials 1 and 3 at the same concentration of 3.125 μg/mL as compared to the high antiproliferative property of positive control, as seen in Trial 2. Data showed that the extract has minimal effectiveness even at 1.56 μg/mL concentration, implying that other extraction techniques such as fractionation and purification may be used to satisfy its antiproliferative property.
The extract contains polyalcohol, phenol, polyphenol, and polyhydroxylated metabolites, which are structures that correspond to the major groups of flavonoids (structures that have antioxidant properties), contributing to the high TFC values.
剑叶龙血树，在菲律宾当地被称为蛇草，在天门冬科劳伦蒂品种下，其特征是具有直立的剑状叶子，边缘呈鲜黄色。这种植物已被确定为癌症研究的一个可行候选对象。
研究剑叶龙血树半纯化甲醇提取物的抗增殖和细胞毒性能力，为癌症研究提供依据。
对植物提取物进行（1）定性植物化学分析，（2）仪器分析，包括傅里叶变换红外光谱（FTIR - ATR）和总黄酮含量（TFC），分别用于量化生物活性成分、分析结构和评估生物化学物质，并对（3）HCT 116人结肠癌细胞系进行MTT细胞毒性试验。
剑叶龙血树提取物显示含有黄酮类、皂苷类、甾醇类、三萜类、生物碱类和糖苷类，所有这些都含有一个OH基团，并且在极性溶剂中具有高溶解度。这与TFC的结果相关，发现其在266.8333 mg - 622.6801 mg范围内，以每毫升微克槲皮素表示，线性方程为y = 0.0005x + 0.023，系数R值为0.9933。这一发现与FTIR - ATR数据相对应，该数据显示在3327.21峰处有突出的宽峰 - OH（伯醇和仲醇）。在MTT细胞毒性试验中，与阿霉素相比，其IC50最小，如试验2中IC50 = 0.8012 μg/mL，而抗增殖活性显示，在试验1和3中，在相同浓度3.125 μg/mL时，剑叶龙血树提取物的抑制活性最小，与阳性对照的高抗增殖特性相比，如试验2所示。数据表明，即使在1.56 μg/mL浓度下，剑叶龙血树提取物的效果也最小，这意味着可能需要使用其他提取技术，如分馏和纯化，来满足其抗增殖特性。
剑叶龙血树提取物含有多元醇、酚、多酚和多羟基化代谢物，这些结构与主要的黄酮类化合物组（具有抗氧化特性的结构）相对应，导致了高TFC值。
