Skendzel L P, Wilcox K R, Edson D C
Am J Clin Pathol. 1983 Oct;80(4 Suppl):594-8.
Data from laboratories participating in the College of American Pathologists Surveys in 1982 provided information on the trends in testing for antibodies to rubella. Methods used by participants included: passive hemagglutination, 29%; latex agglutination card assay test, 25%; hemagglutination inhibition, 19%; enzyme immunosorbent assays, 13%; indirect fluorescent antibody assays, 11%; and radioimmunoassay, 3%. The results from these methods generally agreed well with the standard HI test, particularly for detection of immunity in negative and strongly positive samples. Laboratories should use caution, however, that the tests they use give satisfactory results with low titered sera. Compared to laboratories using the one to three-day-old chick cells with Heparin-MnCl2 for HI, laboratories using Human O Cells tended to get higher titers, and laboratories using Fixed Chick cell or Kaolin methods tended to get lower titers.
来自参与1982年美国病理学家学会调查的实验室的数据提供了有关风疹抗体检测趋势的信息。参与者使用的方法包括：被动血凝试验，29%；乳胶凝集卡片试验，25%；血凝抑制试验，19%；酶免疫吸附试验，13%；间接荧光抗体试验，11%；放射免疫测定，3%。这些方法的结果与标准血凝抑制试验总体上吻合良好，尤其是在检测阴性和强阳性样本中的免疫力时。然而，实验室应谨慎使用所采用的检测方法，确保其在低滴度血清检测中能给出令人满意的结果。与使用含肝素 - 氯化锰的1至3日龄鸡细胞进行血凝抑制试验的实验室相比，使用人O细胞的实验室往往获得更高的滴度，而使用固定鸡细胞或高岭土方法的实验室则往往获得较低的滴度。
