莱姆病：一种用于分离疑似病原体（一种螺旋体）的选择性培养基。
Lyme disease: a selective medium for isolation of the suspected etiological agent, a spirochete.
作者信息
Johnson S E, Klein G C, Schmid G P, Bowen G S, Feeley J C, Schulze T
出版信息
J Clin Microbiol. 1984 Jan;19(1):81-2. doi: 10.1128/jcm.19.1.81-82.1984.
Abstract
A simple procedure with a new selective culture medium for the isolation of the suspected etiological agent of Lyme disease from ticks is described. Live ticks (Ixodes dammini) were ground with a mortar and pestle, and the suspensions were inoculated into a selective and nonselective medium. The selective medium, which contained kanamycin and 5-fluorouracil, yielded positive spirochete cultures from 100% of the pooled ticks and from 79% of the single tick specimens. The isolation rate for the nonselective medium was 0% from the tick pools and 58% from the single tick specimens.
摘要
本文描述了一种使用新型选择性培养基从蜱虫中分离疑似莱姆病病原体的简单方法。将活蜱（达敏硬蜱）用研钵和研杵研磨，然后将悬浮液接种到选择性和非选择性培养基中。含有卡那霉素和5-氟尿嘧啶的选择性培养基，从100%的混合蜱虫样本和79%的单个蜱虫样本中培养出了阳性螺旋体。非选择性培养基从混合蜱虫样本中的分离率为0%，从单个蜱虫样本中的分离率为58%。
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本文引用的文献
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Lyme carditis: cardiac abnormalities of Lyme disease.莱姆病性心脏炎：莱姆病的心脏异常表现。
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Spirochetes isolated from the blood of two patients with Lyme disease.从两名莱姆病患者血液中分离出的螺旋体。
N Engl J Med. 1983 Mar 31;308(13):740-2. doi: 10.1056/NEJM198303313081302.
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The spirochetal etiology of Lyme disease.莱姆病的螺旋体病因
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