Valerius N H, Johansen K S, Nielsen O S, Platz P, Rosenkvist J, Sørensen H
Scand J Haematol. 1981 Jul;27(1):9-18. doi: 10.1111/j.1600-0609.1981.tb00445.x.
Granulocyte transfusions have been complicated by graft-versus-host disease (GVHD) in the recipients. This risk can be eliminated by irradiation of the cell product. The effect of in vitro irradiation on elements of lymphocyte and granulocyte function was therefore studied in order to determine the dose of irradiation which blocked lymphocyte function without affecting the function of granulocytes. Lymphocyte blast transformation after stimulation with mitogens was reduced by 90% after irradiation with 1500 rad and by 97% after 5 000 rad. The response to microbial agents and allogeneic cells was far more radio-sensitive, being completely abolished after irradiation with 1 000 and 500 rad, respectively. Mobility was the function of polymorphonuclear leucocytes (PMN) most affected by irradiation, being slightly but significantly reduced after irradiation with 10 000-20 000 rad. The bactericidal activity was reduced only after irradiation with 40 000 rad or more, while the hexose monophosphate shunt activity and the myeloperoxidase activity were largely unaffected by irradiation with doses of up to 120 000 rad. Hence the results indicate that the irradiation of leucocytes intended for transfusion with a dose of 2 000 rad is likely to prevent GVHD without causing any apparent damage to the PMN.
粒细胞输注曾因受者发生移植物抗宿主病（GVHD）而变得复杂。这种风险可通过对细胞制品进行辐照来消除。因此，研究了体外辐照对淋巴细胞和粒细胞功能成分的影响，以确定能阻断淋巴细胞功能而又不影响粒细胞功能的辐照剂量。用丝裂原刺激后，淋巴细胞的增殖在1500拉德辐照后降低了90%，在5000拉德辐照后降低了97%。对微生物制剂和异基因细胞的反应对辐射更为敏感，分别在1000拉德和500拉德辐照后完全被消除。运动性是多形核白细胞（PMN）受辐照影响最大的功能，在10000 - 20000拉德辐照后略有但显著降低。杀菌活性仅在40000拉德或更高剂量辐照后降低，而磷酸己糖旁路活性和髓过氧化物酶活性在高达120000拉德的剂量辐照下基本不受影响。因此，结果表明，以2000拉德的剂量对用于输血的白细胞进行辐照，可能会预防GVHD，而不会对PMN造成任何明显损害。
