Scott D F, Wood D O, Brownell G H, Carter M J, Best G K
Antimicrob Agents Chemother. 1978 Apr;13(4):641-4. doi: 10.1128/AAC.13.4.641.
Three clinical isolates of Staphylococcus aureus, which were previously shown to contain a 50S plasmid conferring resistance to several aminoglycosides, were examined for modifying enzymes. Both the wild-type and heat-cured derivatives of the isolates were screened for acetyl-, adenylyl-, and phosphotransferase activities. The substrates were gentamicin, amikacin, and netilmicin; the results indicated that even though all three activites were present, the phosphotransferase reaction was most responsible for resistance to these antibiotics. The absence of any of the modifying activites in cured derivatives of the three isolates supports the conclusion that aminoglycoside resistance in these strains is conferred by a plasmid.
对先前已证明含有赋予对多种氨基糖苷类抗生素耐药性的50S质粒的三株金黄色葡萄球菌临床分离株进行了修饰酶检测。对这些分离株的野生型和热固化衍生物都进行了乙酰转移酶、腺苷转移酶和磷酸转移酶活性筛选。底物为庆大霉素、阿米卡星和奈替米星；结果表明，尽管三种活性都存在，但磷酸转移酶反应对这些抗生素的耐药性起主要作用。这三株分离株的固化衍生物中不存在任何修饰活性，这支持了这些菌株中的氨基糖苷类耐药性是由质粒赋予的这一结论。
