Fan X G, Chua A, Fan X J, Keeling P W
Department of Clinical Medicine, St James's Hospital, Trinity College, Dublin, Ireland.
J Clin Pathol. 1995 Feb;48(2):133-6. doi: 10.1136/jcp.48.2.133.
To investigate the role of interleukin-8 (IL-8) and tumour necrosis factor (TNF) in patients infected with Helicobacter pylori.
The study population comprised 52 patients with dyspepsia attending for upper gastrointestinal endoscopy. Of these patients, 35 were infected with H pylori. IL-8 and TNF concentrations in plasma, gastric juice, and gastric biopsy homogenate supernatant fluid were measured by radioimmunoassay and L929 cell bioassay, respectively.
The concentrations of IL-8 and TNF in gastric juice and gastric biopsy homogenates were substantially greater in patients infected with H pylori. In H pylori positive patients IL-8 concentrations in gastric juice and gastric biopsy homogenates were higher in those with moderate gastritis than in those with mild gastritis. There was a positive correlation between IL-8 and TNF concentrations in gastric juice and gastric biopsy homogenate supernatant fluid from H pylori positive patients. There were no significant differences between H pylori positive and negative patients with respect to IL-8 and TNF plasma concentrations.
This study suggests that increased gastric production of IL-8 and TNF may be implicated in the pathogenesis of H pylori associated gastroduodenal disease.
研究白细胞介素-8（IL-8）和肿瘤坏死因子（TNF）在幽门螺杆菌感染患者中的作用。
研究对象包括52例因消化不良前来接受上消化道内镜检查的患者。其中，35例感染了幽门螺杆菌。分别采用放射免疫分析法和L929细胞生物测定法测定血浆、胃液及胃活检匀浆上清液中IL-8和TNF的浓度。
感染幽门螺杆菌的患者胃液及胃活检匀浆中IL-8和TNF的浓度显著更高。在幽门螺杆菌阳性患者中，中度胃炎患者胃液及胃活检匀浆中的IL-8浓度高于轻度胃炎患者。幽门螺杆菌阳性患者胃液及胃活检匀浆上清液中IL-8和TNF的浓度呈正相关。幽门螺杆菌阳性和阴性患者的血浆IL-8和TNF浓度无显著差异。
本研究表明，胃内IL-8和TNF生成增加可能与幽门螺杆菌相关的胃十二指肠疾病发病机制有关。
