Wilske B, Busch U, Fingerle V, Jauris-Heipke S, Preac Mursic V, Rössler D, Will G
Max-von-Pettenkofer Institut für Hygiene und Medizinische Mikrobiologie, München, Germany.
Infection. 1996 Mar-Apr;24(2):208-12. doi: 10.1007/BF01713341.
Borrelia burgdorferi sensu lato, the etiological agent of Lyme borreliosis is considerably heterogeneous in Europe. Since the outer surface proteins OspA and OspC are the most promising candidates for a Borrelia vaccine the immunological heterogeneity of these proteins was investigated. By immunological analysis with monoclonal antibodies and sequence analysis of PCR amplified OspA and OspC at least seven and 16 different types, respectively, were found. Whereas skin isolates (n = 68) were quite homogeneous (84% belonged to OspA-serotype 2 or Borrelia afzelii), isolates from human cerebrospinal fluid and from ticks (n = 43 and n = 90 respectively) were highly heterogeneous in their OspA-serotypes with prevalence of the Borrelia garinii associated types (about 70%). OspA-type 4 was often found among isolates from cerebrospinal fluid (28%). In ticks type 4 OspA has not been detected by culture so far. However, as reported in a previous study, type 4 OspA could be detected in ticks by the highly sensitive PCR technique.
莱姆病疏螺旋体复合群（Borrelia burgdorferi sensu lato）是莱姆病的病原体，在欧洲具有相当大的异质性。由于外表面蛋白OspA和OspC是疏螺旋体疫苗最有前景的候选抗原，因此对这些蛋白的免疫异质性进行了研究。通过单克隆抗体免疫分析以及对PCR扩增的OspA和OspC进行序列分析，分别发现了至少7种和16种不同类型。皮肤分离株（n = 68）相当一致（84%属于OspA血清型2或阿氏疏螺旋体（Borrelia afzelii）），而来自人脑脊液和蜱虫的分离株（分别为n = 43和n = 90）在OspA血清型上高度异质，加氏疏螺旋体（Borrelia garinii）相关类型占优势（约70%）。OspA 4型常在脑脊液分离株中发现（28%）。到目前为止，通过培养在蜱虫中尚未检测到4型OspA。然而，如先前一项研究报道，通过高灵敏度PCR技术可在蜱虫中检测到4型OspA。
