Moen B E, Nilsson R, Nordlinder R, Ovrebø S, Bleie K, Skorve A H, Hollund B E
Division of Occupational Medicine, University of Bergen, Norway.
Occup Environ Med. 1996 Oct;53(10):692-6. doi: 10.1136/oem.53.10.692.
Machinists have an increased risk of lung cancer and bladder cancer, and this may be caused by exposure to carcinogenic compounds such as asbestos and polycyclic aromatic hydrocarbons (PAHs) in the engine room. The aim of this study was to investigate the exposure of engine room personnel to PAHs, with 1-hydroxypyrene in urine as a biomarker.
Urine samples from engine room personnel (n = 51) on 10 ships arriving in different harbours were collected, as well as urine samples from a similar number of unexposed controls (n = 47) on the same ships. Urinary 1-hydroxypyrene was quantitatively measured by high performance liquid chromatography. The exposure to PAHs was estimated by a questionnaire answered by the engine room personnel. On two ships, air monitoring of PAHs in the engine room was performed at sea. Both personal monitoring and area monitoring were performed. The compounds were analysed by gas chromatography of two types (with a flame ionisation detector and with a mass spectrometer).
Significantly more 1-hydroxypyrene was found in urine of personnel who had been working in the engine room for the past 24 hours, than in that of the unexposed seamen. The highest concentrations of 1-hydroxypyrene were found among engine room personnel who had experienced oil contamination of the skin during their work in the engine room. Stepwise logistic regression analysis showed a significant relation between the concentrations of 1-hydroxypyrene, smoking, and estimated exposure to PAHs. No PAHs were detected in the air samples.
Engine room personnel who experience skin exposure to oil and oil products are exposed to PAHs during their work. This indicates that dermal uptake of PAHs is the major route of exposure.
机械师患肺癌和膀胱癌的风险增加，这可能是由于在机舱接触致癌化合物，如石棉和多环芳烃（PAHs）所致。本研究的目的是以尿中1-羟基芘作为生物标志物，调查机舱人员对多环芳烃的接触情况。
收集了抵达不同港口的10艘船上机舱人员（n = 51）的尿液样本，以及同一艘船上数量相近的未接触对照组（n = 47）的尿液样本。采用高效液相色谱法定量测定尿中1-羟基芘。通过机舱人员填写的问卷估计其对多环芳烃的接触情况。在两艘船上，在海上对机舱内的多环芳烃进行了空气监测。同时进行了个人监测和区域监测。通过两种类型的气相色谱法（带火焰离子化检测器和带质谱仪）对化合物进行分析。
在过去24小时内在机舱工作的人员尿液中发现的1-羟基芘明显多于未接触的海员。在机舱工作期间皮肤受到油污污染的机舱人员中，1-羟基芘的浓度最高。逐步逻辑回归分析表明，1-羟基芘浓度、吸烟与估计的多环芳烃接触之间存在显著关系。空气样本中未检测到多环芳烃。
皮肤接触油类和油产品的机舱人员在工作期间接触多环芳烃。这表明经皮肤摄取多环芳烃是主要的接触途径。
