MacDougall J D, Hicks A L, MacDonald J R, McKelvie R S, Green H J, Smith K M
Department of Kinesiology, McMaster University, Hamilton, Ontario, Canada L8S 4K1.
J Appl Physiol (1985). 1998 Jun;84(6):2138-42. doi: 10.1152/jappl.1998.84.6.2138.
Our purpose was to examine the effects of sprint interval training on muscle glycolytic and oxidative enzyme activity and exercise performance. Twelve healthy men (22 +/- 2 yr of age) underwent intense interval training on a cycle ergometer for 7 wk. Training consisted of 30-s maximum sprint efforts (Wingate protocol) interspersed by 2-4 min of recovery, performed three times per week. The program began with four intervals with 4 min of recovery per session in week 1 and progressed to 10 intervals with 2.5 min of recovery per session by week 7. Peak power output and total work over repeated maximal 30-s efforts and maximal oxygen consumption (VO2 max) were measured before and after the training program. Needle biopsies were taken from vastus lateralis of nine subjects before and after the program and assayed for the maximal activity of hexokinase, total glycogen phosphorylase, phosphofructokinase, lactate dehydrogenase, citrate synthase, succinate dehydrogenase, malate dehydrogenase, and 3-hydroxyacyl-CoA dehydrogenase. The training program resulted in significant increases in peak power output, total work over 30 s, and VO2 max. Maximal enzyme activity of hexokinase, phosphofructokinase, citrate synthase, succinate dehydrogenase, and malate dehydrogenase was also significantly (P < 0.05) higher after training. It was concluded that relatively brief but intense sprint training can result in an increase in both glycolytic and oxidative enzyme activity, maximum short-term power output, and VO2 max.
我们的目的是研究短跑间歇训练对肌肉糖酵解和氧化酶活性以及运动表现的影响。12名健康男性（年龄22±2岁）在功率自行车上进行了7周的高强度间歇训练。训练包括30秒的最大冲刺努力（温盖特方案），中间穿插2 - 4分钟的恢复时间，每周进行三次。该计划从第1周每次训练4个间歇，每个间歇恢复4分钟开始，到第7周进展到每次训练10个间歇，每个间歇恢复2.5分钟。在训练计划前后测量了重复30秒最大努力时的峰值功率输出、总功以及最大摄氧量（VO2 max）。在训练计划前后，从9名受试者的股外侧肌取针吸活检样本，测定己糖激酶、总糖原磷酸化酶、磷酸果糖激酶、乳酸脱氢酶、柠檬酸合酶、琥珀酸脱氢酶、苹果酸脱氢酶和3 - 羟基酰基辅酶A脱氢酶的最大活性。训练计划导致峰值功率输出、30秒内的总功和VO2 max显著增加。训练后，己糖激酶、磷酸果糖激酶、柠檬酸合酶、琥珀酸脱氢酶和苹果酸脱氢酶的最大酶活性也显著（P < 0.05）升高。得出的结论是，相对短暂但高强度的短跑训练可导致糖酵解和氧化酶活性增加、最大短期功率输出增加以及VO2 max增加。
