Ichikawa H, Kuroiwa T, Inagaki A, Shineha R, Nishihira T, Satomi S, Sakata T
Second Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Dig Dis Sci. 1999 Oct;44(10):2119-23. doi: 10.1023/a:1026647024077.
Probiotics are used for various intestinal diseases. However, their effects on gut epithelial cell proliferation have not been investigated. We administered 10(7) colony-forming units of Lactobacillus casei or Clostridium butyricum, or no probiotics (control) by gastric intubation once a day for seven days to rats fed an elemental diet. We estimated the crypt cell production rate of the jejunum, ileum, cecum, and distal colon. We also quantified cecal bacteria. Both probiotics increased the crypt cell production rate of the jejunum and ileum by 25-40%, of the cecum by 70%, and of the distal colon by more than 200% compared with control. Only minor variance in the cecal bacterial composition existed among the three groups. Probiotics enhanced gut epithelial cell proliferation in rats fed an elemental diet.
益生菌被用于治疗各种肠道疾病。然而，它们对肠道上皮细胞增殖的影响尚未得到研究。我们通过胃管每天给食用要素饮食的大鼠灌胃10⁷ 个菌落形成单位的干酪乳杆菌或丁酸梭菌，或不给予益生菌（对照），持续7天。我们估计了空肠、回肠、盲肠和远端结肠的隐窝细胞产生率。我们还对盲肠细菌进行了定量分析。与对照组相比，两种益生菌均使空肠和回肠的隐窝细胞产生率提高了25%-40%，使盲肠的隐窝细胞产生率提高了70%，使远端结肠的隐窝细胞产生率提高了200%以上。三组之间盲肠细菌组成仅存在微小差异。益生菌可增强食用要素饮食大鼠的肠道上皮细胞增殖。
