用于白色念珠菌和其他酵母菌的菌株分型、药敏试验及其他生理测量的带有标准化接种物的定量微培养系统。
Quantitative microculture system with standardized inocula for strain typing, susceptibility testing, and other physiologic measurements with Candida albicans and other yeasts.
作者信息
Odds F C
机构信息
Department of Bacteriology and Mycology, Janssen Research Foundation, Beerse, Belgium.
出版信息
J Clin Microbiol. 1991 Dec;29(12):2735-40. doi: 10.1128/jcm.29.12.2735-2740.1991.
Abstract
A method for physiologic testing of Candida albicans and other yeasts is described in which inocula with reproducible cell concentrations are prepared from cultures in a glucose-limited medium and growth turbidities of test cultures in microplates are expressed quantitatively as a percentage of control growth. The method gave good within-plate and run-to-run reproducibilities and was applicable both to strain phenotype discrimination in C. albicans and to susceptibility tests of yeasts with azole antifungal agents.
摘要
本文描述了一种白色念珠菌和其他酵母菌的生理学测试方法，该方法通过在葡萄糖限制培养基中培养来制备具有可重复细胞浓度的接种物，并将微孔板中测试培养物的生长浊度定量表示为对照生长的百分比。该方法在板内和不同批次间具有良好的重复性，适用于白色念珠菌菌株表型鉴别以及酵母菌对唑类抗真菌药物的药敏试验。
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Quantitative microculture system with standardized inocula for strain typing, susceptibility testing, and other physiologic measurements with Candida albicans and other yeasts.用于白色念珠菌和其他酵母菌的菌株分型、药敏试验及其他生理测量的带有标准化接种物的定量微培养系统。
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本文引用的文献
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Relative inhibition factors--a novel approach to the assessment of antifungal antibiotics in vitro.相对抑制因子——体外评估抗真菌抗生素的一种新方法。
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Antifungal susceptibility tests.抗真菌药敏试验。
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Improved method for azole antifungal susceptibility testing.改进的唑类抗真菌药敏试验方法。
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[Comparative sensitivity of yeasts to ketoconazole, itraconazole and fluconazole using a liquid medium standardized micromethod].[使用标准化微量液体培养基法比较酵母菌对酮康唑、伊曲康唑和氟康唑的敏感性]
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