Lee Kiyoung, Choo Yoe-Jin, Giovannoni Stephen J, Cho Jang-Cheon
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA.
Int J Syst Evol Microbiol. 2007 Jul;57(Pt 7):1653-1658. doi: 10.1099/ijs.0.64960-0.
A Gram-negative, chemoheterotrophic, strictly aerobic, alkaliphilic, rod-shaped marine bacterium, designated HTCC2654(T), was isolated from the western Sargasso Sea by using a dilution-to-extinction culturing method. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HTCC2654(T) belonged to the Roseobacter clade of the order Rhodobacterales. The 16S rRNA gene sequence similarity of the strain with respect to other members of the Roseobacter clade ranged from 90.4 to 95.1 %. In the phylogenetic analyses, the strain formed an independent phyletic line and could not be assigned to any other known genera of the Rhodobacterales. The DNA G+C content of strain HTCC2654(T) was 61.7 mol% by HPLC and 64.1 mol% from genome sequences. The predominant constituents of the cellular fatty acids were C(16 : 0) 2-OH (27.3 %), 11-methyl C(18 : 1) omega 7c (19.6 %) and C(18 : 1) omega 7c (17.3 %), and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine, which served to differentiate the strain from other members of the Roseobacter clade. On the basis of the taxonomic data obtained in this study, strain HTCC2654(T) represents a novel genus and species, for which the name Maritimibacter alkaliphilus gen. nov., sp. nov. is proposed. The type strain is HTCC2654(T) (=KCCM 42376(T)=NBRC 102057(T)).
采用稀释至灭绝培养法从马尾藻海西部分离出一株革兰氏阴性、化能异养、严格需氧、嗜碱、杆状的海洋细菌，命名为HTCC2654(T)。基于16S rRNA基因序列的系统发育分析表明，菌株HTCC2654(T)属于红杆菌目红杆菌科。该菌株与红杆菌科其他成员的16S rRNA基因序列相似性在90.4%至95.1%之间。在系统发育分析中，该菌株形成了一条独立的进化线，不能归属于红杆菌目的任何其他已知属。通过高效液相色谱法测定，菌株HTCC2654(T)的DNA G+C含量为61.7 mol%，基因组序列测定结果为64.1 mol%。细胞脂肪酸的主要成分是C(16 : 0) 2-OH（27.3%）、11-甲基C(18 : 1) ω7c（19.6%）和C(18 : 1) ω7c（17.3%），主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱，这些成分可将该菌株与红杆菌科的其他成员区分开来。根据本研究获得的分类学数据，菌株HTCC2654(T)代表一个新的属和种，提议将其命名为嗜碱海洋杆菌属（Maritimibacter alkaliphilus），新属，新种。模式菌株为HTCC2654(T)（=KCCM 42376(T)=NBRC 102057(T)）。
