Chakrabarti A, Nayak N, Talwar P
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Mycopathologia. 1991 Jun;114(3):163-8. doi: 10.1007/BF00437209.
A total of 290 Candida isolates from patients were investigated for in vitro proteinase production. Overall, sixty percent of these strains were found to be proteinase producers. Of the C. albicans strains, 81.4% of the significant isolates in contrast to 19.7% of nonsignificant isolates were proteinase producers, the difference being statistically significant (P less than 0.001). Amongst the different Candida species, the proteinase production was found not only in Candida albicans, but also in C. tropicalis, C. parapsilosis and C. glabrata. Thus this in vitro method of demonstration of proteinase may be a good adjunct to smear and culture examination in identifying pathogenic Candida species from anatomical sites where they can also be present as commensals.
对从患者身上分离出的290株念珠菌进行了体外蛋白酶产生情况的研究。总体而言，发现这些菌株中有60%是蛋白酶产生菌。在白色念珠菌菌株中，81.4%的显著分离株是蛋白酶产生菌，而非显著分离株中这一比例为19.7%，差异具有统计学意义（P小于0.001）。在不同的念珠菌物种中，不仅在白色念珠菌中发现了蛋白酶产生，在热带念珠菌、近平滑念珠菌和光滑念珠菌中也有发现。因此，这种体外蛋白酶检测方法可能是涂片和培养检查的良好辅助手段，有助于从解剖部位识别致病性念珠菌物种，因为在这些部位它们也可能作为共生菌存在。
