Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, PR China.
Graduate University, CAS, Beijing 100049, PR China.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1807-1812. doi: 10.1099/ijs.0.017053-0. Epub 2009 Sep 18.
An actinomycete, designated MS426T, the culture broth of which showed potent antimicrobial activity, was isolated from a deep-sea sediment sample of the South China Sea. An almost-complete sequence of the 16S rRNA gene of strain MS426T was determined and aligned with those of representatives of the family Micromonosporaceae available in public databases. Phylogenetic trees were inferred by using three algorithms. Strain MS426T formed a branch adjacent to Verrucosispora lutea YIM 013T in a distinct cluster occupied only by strains of the genus Verrucosispora. Strain MS426T was distinguishable from the type strains of the two described Verrucosispora species by using a combination of chemical and morphological markers and by DNA-DNA relatedness. On the basis of these genotypic and phenotypic differences, the novel antimicrobial strain with pharmaceutical potential represents a novel species, for which the name Verrucosispora sediminis sp. nov. is proposed. The type strain is MS426T (=CGMCC 4.3550T =JCM 15670T).
从南海深海沉积物样本中分离到一株放线菌 MS426T，其发酵液表现出很强的抗菌活性。测定了菌株 MS426T 的 16S rRNA 基因的近乎完整序列，并与公共数据库中可获得的微单孢菌科代表菌株进行了比对。通过使用三种算法构建了系统发育树。MS426T 菌株与黄颜色放线菌 YIM 013T 形成一个分支，该分支位于一个独特的分支中，仅由疣孢菌属的菌株占据。MS426T 菌株可通过化学和形态标记以及 DNA-DNA 同源性与两种已描述的疣孢菌的模式菌株相区别。基于这些基因型和表型差异，具有药物潜力的新型抗菌菌株代表了一个新的物种，建议将其命名为深海疣孢菌（Verrucosispora sediminis）sp. nov.。其模式菌株为 MS426T（=CGMCC 4.3550T=JCM 15670T）。
