超灵敏酶促放射免疫测定法：应用于霍乱毒素和轮状病毒的检测
Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.
作者信息
Harris C C, Yolken R H, Krokan H, Hsu I C
出版信息
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5336-9. doi: 10.1073/pnas.76.10.5336.
Abstract
Rotavirus and enterotoxin-producing bacteria are major causes of diarrheal disease in humans. A method of rapid diagnosis, ultrasensitive enzymatic radioimmunoassay, has been developed to quantitatively detect cholera toxin and rotavirus. The method uses features of both enzyme-linked immunosorbent assay and radioimmunoassay; however, the sensitivity of the assay is 100- to 1000-fold more sensitive than the two parent assays. Ultrasensitive enzymatic radioimmunoassay should also be useful in measuring other biologically important agents such as drugs and hormones.
摘要
轮状病毒和产肠毒素细菌是人类腹泻疾病的主要病因。一种快速诊断方法——超灵敏酶免疫测定法已被开发出来，用于定量检测霍乱毒素和轮状病毒。该方法兼具酶联免疫吸附测定法和放射免疫测定法的特点；然而，该测定法的灵敏度比两种原始测定法高100至1000倍。超灵敏酶免疫测定法在测量其他生物重要物质如药物和激素方面也应很有用。
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Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.超灵敏酶促放射免疫测定法：应用于霍乱毒素和轮状病毒的检测
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