小鼠巨细胞病毒与分离的脾细胞群体的相互作用。
Interaction of murine cytomegalovirus with separated populations of spleen cells.
作者信息
Loh L, Hudson J B
出版信息
Infect Immun. 1979 Dec;26(3):853-60. doi: 10.1128/iai.26.3.853-860.1979.
Abstract
Mouse spleen cultures were infected with murine cytomegalovirus, either before or after separating the cells into various classes, and the growth of virus and production of infectious centers were measured in each cell class. The separation techniques, which were used in various combinations, comprised: adherence to plastic surfaces; adherence to nylon wool columns; gamma irradiation; and treatment with anti-immunoglobulin serum plus complement. The "macrophage population" took up most of the input virus, as shown by autoradiography of spleen cells infected with radioactive virus. These cells formed infectious centers but replicated the virus only transiently. In contrast, the "B-lymphocyte population" contained a minor cell fraction which was permissive for replication. The "T-lymphocyte population" appeared to be nonpermissive for replication and did not produce infectious centers.
摘要
在将小鼠脾脏细胞分为不同类别之前或之后，用鼠巨细胞病毒感染小鼠脾脏培养物，并测定每个细胞类别中病毒的生长情况和感染中心的产生情况。所使用的分离技术以各种组合方式进行，包括：贴附于塑料表面；贴附于尼龙毛柱；γ射线照射；以及用抗免疫球蛋白血清加补体处理。如对感染放射性病毒的脾脏细胞进行放射自显影所示，“巨噬细胞群体”摄取了大部分输入病毒。这些细胞形成感染中心，但仅短暂复制病毒。相比之下，“B淋巴细胞群体”含有一小部分允许病毒复制的细胞。“T淋巴细胞群体”似乎不允许病毒复制，也不产生感染中心。
相似文献
1
Interaction of murine cytomegalovirus with separated populations of spleen cells.
Infect Immun. 1979 Dec;26(3):853-60. doi: 10.1128/iai.26.3.853-860.1979.
2
Spontaneous activation of latent cytomegalovirus from murine spleen explants. Role of lymphocytes and macrophages in release and replication of virus.
J Clin Invest. 1982 Oct;70(4):762-8. doi: 10.1172/jci110672.
3
Replication of murine leukemia virus in bone marrow-derived lymphocytes.
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3714-8. doi: 10.1073/pnas.73.10.3714.
4
Studies of the producer cell of herpes simplex virus-induced interferon in mouse spleen cell cultures.
Immunobiology. 1980 Dec;157(4-5):401-6. doi: 10.1016/S0171-2985(80)80009-X.
5
In vitro studies of the rabbit immune system: II. Functional characterization of rabbit T and B populations separated by adherence to nylon wool or lysis with anti-thymocyte serum and complement.
Cell Immunol. 1976 Jun 1;24(1):11-23. doi: 10.1016/0008-8749(76)90127-1.
6
Nylon wool column separation of chicken thymic-derived (T) and bursal-derived (B) lymphocytes.
Poult Sci. 1978 Mar;57(2):518-22. doi: 10.3382/ps.0570518.
7
Target cell heterogeneity in murine leukemia virus infection. II. Demonstration of Friend leukemia-virus-permissive and non-permissive subsets of splenic T cells.
Int J Cancer. 1981;27(4):505-12. doi: 10.1002/ijc.2910270414.
8
Mouse hepatitis virus 3 replication in T and B lymphocytes correlate with viral pathogenicity.
J Immunol. 1989 Jun 15;142(12):4458-65.
9
Persistence and expression of herpes virus in guinea pig B and T spleen cells.
Proc Soc Exp Biol Med. 1979 Oct;162(1):202-6. doi: 10.3181/00379727-162-40647.
10
Interactions of murine leukemia virus (MuLV) with isolated lymphocytes. II. Infections of B and T cells with Friend virus complex indiffusion chambers and in vitro: effect of polyclonal mitogens.
Int J Cancer. 1976 Aug 15;18(2):189-96. doi: 10.1002/ijc.2910180208.
引用本文的文献
1
Suppressor of Cytokine Signaling 1 (SOCS1) and SOCS3 Are Stimulated within the Eye during Experimental Murine Cytomegalovirus Retinitis in Mice with Retrovirus-Induced Immunosuppression.
J Virol. 2018 Aug 29;92(18). doi: 10.1128/JVI.00526-18. Print 2018 Sep 15.
2
HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species.
BMC Biotechnol. 2016 Jan 8;16:2. doi: 10.1186/s12896-016-0232-6.
3
Murine peritoneal macrophages support murine cytomegalovirus replication.
Infect Immun. 1983 Sep;41(3):1352-9. doi: 10.1128/iai.41.3.1352-1359.1983.
4
Murine cytomegalovirus-induced immunosuppression.
Infect Immun. 1982 Apr;36(1):89-95. doi: 10.1128/iai.36.1.89-95.1982.
5
Effect of murine cytomegalovirus infection on mitogen responses in genetically resistant and susceptible mice.
Infect Immun. 1982 Apr;36(1):235-42. doi: 10.1128/iai.36.1.235-242.1982.
6
Murine cytomegalovirus infection: hematological, morphological, and functional study of lymphoid cells.
Infect Immun. 1981 Jul;33(1):239-49. doi: 10.1128/iai.33.1.239-249.1981.
7
Murine cytomegalovirus infection in the spleen and its relationship to immunosuppression.
Infect Immun. 1981 Jun;32(3):1067-72. doi: 10.1128/iai.32.3.1067-1072.1981.
8
Immunosuppressive effect of murine cytomegalovirus.
Infect Immun. 1980 Jan;27(1):54-60. doi: 10.1128/iai.27.1.54-60.1980.
9
Animal viruses of economic importance: genetic variation, persistence, and prospects for their control.
Pharmacol Ther. 1985;30(2):115-226. doi: 10.1016/0163-7258(85)90033-6.
10
Studies of depressed interleukin-2 production by spleen cells from mice following infection with cytomegalovirus.
Arch Virol. 1988;99(1-2):1-8. doi: 10.1007/BF01311018.
本文引用的文献
1
Cytochemical identification of monocytes and granulocytes.
Am J Clin Pathol. 1971 Mar;55(3):283-90. doi: 10.1093/ajcp/55.3.283.
2
Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo.
J Immunol. 1974 Oct;113(4):1093-7.
3
A rapid method for the isolation of functional thymus-derived murine lymphocytes.
Eur J Immunol. 1973 Oct;3(10):645-9. doi: 10.1002/eji.1830031011.
4
Induction of disseminated virulent cytomegalovirus infection by immunosuppression of naturally chronically infected wild mice.
Infect Immun. 1974 Oct;10(4):966-9. doi: 10.1128/iai.10.4.966-969.1974.
5
Mouse cytomegalovirus: isolation from spleen and lymph nodes of chronically infected mice.
Proc Soc Exp Biol Med. 1972 Jul;140(3):802-6. doi: 10.3181/00379727-140-36556.
6
Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation.
J Virol. 1968 Sep;2(9):853-8. doi: 10.1128/JVI.2.9.853-858.1968.
7
Infection of adult mouse macrophages in vitro with cytomegalovirus.
Proc Soc Exp Biol Med. 1968 Dec;129(3):690-4. doi: 10.3181/00379727-129-33399.
8
Separation of murine lymphoid cells using nylon wool columns. Recovery of the B cell-enriched population.
Transplantation. 1974 Dec;18(6):544-8. doi: 10.1097/00007890-197412000-00013.
9
Role of macrophages in resistance to murine cytomegalovirus.
Infect Immun. 1974 Dec;10(6):1383-90. doi: 10.1128/iai.10.6.1383-1390.1974.
10
Some properties of the genome of murine cytomegalovirus (MCV).
Virology. 1973 Jul;54(1):135-49. doi: 10.1016/0042-6822(73)90123-2.
Zotero 插件