Teets Frank D, Ramgopal Moti N, Sweeney Kristen D, Graham Amanda S, Michael Scott F, Isern Sharon
Department of Biological Sciences, College of Arts and Sciences, Florida Gulf Coast University, 10501 FGCU Boulevard South, Fort Myers, FL 33965.
Martin Health System Center for Clinical Research, 10000 SW Innovation Way, Port St. Lucie, FL 34987.
Virol Rep. 2014 March-June;1-2:2-8. doi: 10.1016/j.virep.2014.05.001.
After a 75-year absence from Florida, substantial local transmission of dengue virus (DENV) occurred in Key West, Monroe County, Florida in 2009 and continued in 2010. The outbreak culminated in 85 reported cases. In 2011 and 2012, only isolated cases of local DENV transmission were reported in Florida, none were reported in Key West. In 2013, a new outbreak occurred, but this time in Martin County about 275 miles North of Key West with 22 reported cases. As the Key West and Martin County outbreaks involved DENV serotype 1 (DENV-1), we wanted to investigate whether the same strain or a different strain of DENV was responsible for the outbreaks. In this study, we report the sequence and phylogenetic analysis of the E generegion from a patient diagnosed with dengue in Martin County. Our results indicate that the 2013 Martin County DENV-1 strain is distinct from the 2009-2010 Key West DENV-1 and that it is most closely related to viruses from a recent expansion of South American DENV-1 strains into the Caribbean. We conclude that the 2013 Martin County outbreak was the result of a new introduction of DENV-1 in Florida.
在佛罗里达州阔别登革热病毒（DENV）75年后，2009年门罗县基韦斯特出现了该病毒的大量本地传播，并在2010年持续。此次疫情最终报告了85例病例。2011年和2012年，佛罗里达州仅报告了个别本地DENV传播病例，基韦斯特未报告任何病例。2013年，又爆发了一次疫情，但这次是在基韦斯特以北约275英里处的马丁县，报告了22例病例。由于基韦斯特和马丁县的疫情都涉及DENV血清型1（DENV-1），我们想调查引发疫情的是同一毒株还是不同的DENV毒株。在本研究中，我们报告了对一名马丁县登革热确诊患者E基因区域的序列和系统发育分析。我们的结果表明，2013年马丁县的DENV-1毒株与2009 - 2010年基韦斯特的DENV-1毒株不同，且与最近从南美洲扩展到加勒比地区的DENV-1毒株关系最为密切。我们得出结论，2013年马丁县的疫情是佛罗里达州新引入DENV-1的结果。
