Chiu Charles Y, Bres Vanessa, Yu Guixia, Krysztof David, Naccache Samia N, Lee Deanna, Pfeil Jacob, Linnen Jeffrey M, Stramer Susan L
Emerg Infect Dis. 2015 Aug;21(8):1409-13. doi: 10.3201/eid2108.150458.
A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014-2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.
在2014 - 2015年加勒比地区疫情期间,一种新开发的转录介导扩增检测方法被用于检测来自波多黎各的557名无症状献血者中的3人(0.54%)是否感染基孔肯雅病毒。通过使用聚合酶链反应(PCR)、微阵列和下一代测序技术对病毒检测结果进行了确认。分子钟分析将波多黎各毒株的出现时间追溯到2013年初。