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2014年波多黎各用于鉴定献血者中基孔肯雅病毒的基因组检测方法

Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014.

作者信息

Chiu Charles Y, Bres Vanessa, Yu Guixia, Krysztof David, Naccache Samia N, Lee Deanna, Pfeil Jacob, Linnen Jeffrey M, Stramer Susan L

出版信息

Emerg Infect Dis. 2015 Aug;21(8):1409-13. doi: 10.3201/eid2108.150458.

DOI:10.3201/eid2108.150458
PMID:26196378
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4517739/
Abstract

A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014-2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.

摘要

在2014 - 2015年加勒比地区疫情期间，一种新开发的转录介导扩增检测方法被用于检测来自波多黎各的557名无症状献血者中的3人（0.54%）是否感染基孔肯雅病毒。通过使用聚合酶链反应（PCR）、微阵列和下一代测序技术对病毒检测结果进行了确认。分子钟分析将波多黎各毒株的出现时间追溯到2013年初。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/4517739/8e7dd6cb2420/15-0458-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/4517739/41aa3c88e080/15-0458-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/4517739/8e7dd6cb2420/15-0458-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/4517739/41aa3c88e080/15-0458-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3afd/4517739/8e7dd6cb2420/15-0458-F2.jpg

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