• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种用于大鼠器官组织中过氧化氢水平成像的双光子比率荧光探针。

A Two-Photon Ratiometric Fluorescent Probe for Imaging of Hydrogen Peroxide Levels in Rat Organ Tissues.

作者信息

Lim Chang Su, Cho Myoung Ki, Park Mi Yeon, Kim Hwan Myung

机构信息

Department of Energy System Research and Department of Chemistry Ajou University Suwon Gyeonggi-do 443-749 Republic of Korea.

出版信息

ChemistryOpen. 2017 Nov 22;7(1):53-56. doi: 10.1002/open.201700155. eCollection 2018 Jan.

DOI:10.1002/open.201700155
PMID:29318096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5754560/
Abstract

Hydrogen peroxide (HO) is important in the regulation of a variety of biological processes and is involved in various diseases. Quantitative measurement of HO levels at the subcellular level is important for understanding its positive and negative effects on biological processes. Herein, a two-photon ratiometric fluorescent probe (SHP-Cyto) with a boronate-based carbamate leaving group as the HO reactive trigger and 6-(benzo[]thiazol-2'-yl)-2-(,-dimethylamino) naphthalene (BTDAN) as the fluorophore was synthesized and examined for its ability to detect cytosolic HO in situ. This probe, based on the specific reaction between boronate and HO, displayed a fluorescent color change (455 to 528 nm) in response to HO in the presence of diverse reactive oxygen species in a physiological medium. In addition, ratiometric two-photon microscopy (TPM) images with SHP-Cyto revealed that HO levels gradually increased from brain to kidney, skin, heart, lung, and then liver tissues. SHP-Cyto was successfully applied to the imaging of endogenously produced cytosolic HO levels in live cells and various rat organs by using TPM.

摘要

过氧化氢（HO）在多种生物过程的调节中起着重要作用，并与多种疾病相关。在亚细胞水平上对HO水平进行定量测量对于理解其对生物过程的正负影响至关重要。在此，合成了一种双光子比率荧光探针（SHP-Cyto），其以基于硼酸酯的氨基甲酸酯离去基团作为HO反应触发基团，以6-(苯并[]噻唑-2'-基)-2-(,-二甲基氨基)萘（BTDAN）作为荧光团，并检测了其原位检测胞质HO的能力。该探针基于硼酸酯与HO之间的特异性反应，在生理介质中存在多种活性氧的情况下，对HO呈现出荧光颜色变化（从455 nm到528 nm）。此外，使用SHP-Cyto的比率双光子显微镜（TPM）图像显示，HO水平从脑组织到肾脏、皮肤、心脏、肺，然后到肝脏组织逐渐升高。通过TPM，SHP-Cyto成功应用于活细胞和各种大鼠器官中内源性产生的胞质HO水平的成像。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/fb258171e3d4/OPEN-7-53-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/3ac92b08733b/OPEN-7-53-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/e1f313189731/OPEN-7-53-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/261e366cff73/OPEN-7-53-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/e24a4bdf095b/OPEN-7-53-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/fb258171e3d4/OPEN-7-53-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/3ac92b08733b/OPEN-7-53-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/e1f313189731/OPEN-7-53-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/261e366cff73/OPEN-7-53-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/e24a4bdf095b/OPEN-7-53-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43b5/5754560/fb258171e3d4/OPEN-7-53-g004.jpg

相似文献

1
A Two-Photon Ratiometric Fluorescent Probe for Imaging of Hydrogen Peroxide Levels in Rat Organ Tissues.一种用于大鼠器官组织中过氧化氢水平成像的双光子比率荧光探针。
ChemistryOpen. 2017 Nov 22;7(1):53-56. doi: 10.1002/open.201700155. eCollection 2018 Jan.
2
Recent advances in hydrogen peroxide imaging for biological applications.用于生物应用的过氧化氢成像的最新进展。
Cell Biosci. 2014 Oct 27;4(1):64. doi: 10.1186/2045-3701-4-64. eCollection 2014.
3
Synthesis and application of a ratiometric probe for hydrogen peroxide.一种用于过氧化氢的比率型探针的合成与应用。
Methods Enzymol. 2020;639:23-36. doi: 10.1016/bs.mie.2020.04.007. Epub 2020 Apr 29.
4
A red-emitting styrylnaphthalimide-based fluorescent probe providing a ratiometric signal change for the precise and quantitative detection of HO.一种基于红色发射的苯乙烯萘酰亚胺的荧光探针，提供了一种比率信号变化，可用于精确和定量检测 HO。
Anal Chim Acta. 2019 Nov 8;1080:153-161. doi: 10.1016/j.aca.2019.07.008. Epub 2019 Jul 9.
5
A new mitochondria-targeting fluorescent probe for ratiometric detection of HO in live cells.一种新的线粒体靶向荧光探针，用于活细胞中 HO 的比率检测。
Anal Chim Acta. 2020 Feb 8;1097:230-237. doi: 10.1016/j.aca.2019.11.024. Epub 2019 Nov 18.
6
A pyrene-based ratiometric fluorescent probe with a large Stokes shift for selective detection of hydrogen peroxide in living cells.一种基于芘的具有大斯托克斯位移的比率型荧光探针，用于在活细胞中选择性检测过氧化氢。
J Pharm Anal. 2020 Oct;10(5):490-497. doi: 10.1016/j.jpha.2020.07.003. Epub 2020 Aug 4.
7
Development of a ratiometric two-photon fluorescent probe for imaging of hydrogen peroxide in ischemic brain injury.用于成像缺血性脑损伤中过氧化氢的比率型双光子荧光探针的研制。
Analyst. 2019 Oct 7;144(20):5965-5970. doi: 10.1039/c9an01326a.
8
A two-photon NIR-to-NIR fluorescent probe for imaging hydrogen peroxide in living cells.用于在活细胞中成像过氧化氢的双光子近红外到近红外荧光探针。
Biosens Bioelectron. 2017 Aug 15;94:536-543. doi: 10.1016/j.bios.2017.03.039. Epub 2017 Mar 19.
9
Construction of a lysosome-targetable ratiometric fluorescent probe for HO tracing and imaging in living cells and an inflamed model.构建一种可靶向溶酶体的比率荧光探针，用于在活细胞和炎症模型中追踪和成像HO。
RSC Adv. 2021 Jul 7;11(39):24032-24037. doi: 10.1039/d1ra04026j. eCollection 2021 Jul 6.
10
A mitochondria targetable two-photon excited near-infrared fluorescent probe for imaging of HO in live cells and tissues.一种线粒体靶向的双光子激发近红外荧光探针，用于活细胞和组织中 HO 的成像。
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 5;206:529-534. doi: 10.1016/j.saa.2018.08.042. Epub 2018 Aug 25.

引用本文的文献

1
Diverse Fluorescent Probe Concepts for Detection and Monitoring of Reactive Oxygen Species.用于检测和监测活性氧物种的多种荧光探针概念
Chem Asian J. 2025 Apr 3;20(7):e202401524. doi: 10.1002/asia.202401524. Epub 2025 Feb 20.
2
Boronates as hydrogen peroxide-reactive warheads in the design of detection probes, prodrugs, and nanomedicines used in tumors and other diseases.硼酸酯作为过氧化氢反应性弹头，用于肿瘤及其他疾病的检测探针、前药和纳米药物的设计中。
Drug Deliv Transl Res. 2023 May;13(5):1305-1321. doi: 10.1007/s13346-022-01248-w. Epub 2022 Oct 18.
3
Research progress in the development of organic small molecule fluorescent probes for detecting HO.

本文引用的文献

1
A novel method for the synthesis of 1,2-benzisoxazoline-3-one and its application to hypochlorite recognition.一种合成1,2-苯并异恶唑啉-3-酮的新方法及其在次氯酸盐识别中的应用。
J Mater Chem B. 2016 Aug 14;4(30):5101-5104. doi: 10.1039/c6tb01392a. Epub 2016 Jul 14.
2
A carboxylesterase-selective ratiometric fluorescent two-photon probe and its application to hepatocytes and liver tissues.一种羧酸酯酶选择性比率荧光双光子探针及其在肝细胞和肝组织中的应用。
Chem Sci. 2016 Jun 1;7(6):3703-3709. doi: 10.1039/c5sc05001d. Epub 2016 Feb 23.
3
A ratiometric two-photon probe for Ca in live tissues and its application to spinal cord injury model.
用于检测羟基自由基的有机小分子荧光探针的研究进展
RSC Adv. 2019 Jun 7;9(31):18027-18041. doi: 10.1039/c9ra02467k. eCollection 2019 Jun 4.
4
Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology.用于生物氧化剂的硼酸酯基探针：一类用于氧化还原生物学的新型分子工具。
Front Chem. 2020 Sep 25;8:580899. doi: 10.3389/fchem.2020.580899. eCollection 2020.
用于活组织中钙离子的比率型双光子探针及其在脊髓损伤模型中的应用。
Biomaterials. 2017 Oct;141:251-259. doi: 10.1016/j.biomaterials.2017.07.006. Epub 2017 Jul 6.
4
Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues.高选择性和高灵敏度的内源性过氧亚硝酸盐双光子荧光探针及其在活细胞和组织中的应用。
Anal Chem. 2017 Aug 15;89(16):8496-8500. doi: 10.1021/acs.analchem.7b02059. Epub 2017 Jul 24.
5
An -nitrosation reactivity-based two-photon fluorescent probe for the specific detection of nitric oxide.一种基于亚硝化反应活性的双光子荧光探针，用于一氧化氮的特异性检测。
Chem Sci. 2017 Jun 1;8(6):4533-4538. doi: 10.1039/c7sc00416h. Epub 2017 Apr 20.
6
Reversible Ratiometric Fluorescent Probe for Sensing Bisulfate/HO and Its Application in Zebrafish.用于检测亚硫酸氢根/HO 的可逆比率荧光探针及其在斑马鱼中的应用。
Anal Chem. 2017 Aug 1;89(15):8079-8083. doi: 10.1021/acs.analchem.7b01580. Epub 2017 Jul 12.
7
Selective Visualization of the Endogenous Peroxynitrite in an Inflamed Mouse Model by a Mitochondria-Targetable Two-Photon Ratiometric Fluorescent Probe.线粒体靶向双光子比率荧光探针选择性可视化炎症小鼠模型中的内源性过氧亚硝酸盐。
J Am Chem Soc. 2017 Jan 11;139(1):285-292. doi: 10.1021/jacs.6b10508. Epub 2016 Dec 20.
8
Single Fluorescent Probe for Dual-Imaging Viscosity and HO in Mitochondria with Different Fluorescence Signals in Living Cells.用于在活细胞中对线粒体黏度和羟基自由基进行双成像的单一荧光探针，具有不同荧光信号。
Anal Chem. 2017 Jan 3;89(1):552-555. doi: 10.1021/acs.analchem.6b04385. Epub 2016 Dec 13.
9
Boronate based fluorescence (ESIPT) probe for peroxynitrite.用于过氧亚硝酸盐的基于硼酸盐的荧光（ESIPT）探针。
Chem Commun (Camb). 2016 Oct 11;52(83):12350-12352. doi: 10.1039/c6cc06829d.
10
Rational Design of an α-Ketoamide-Based Near-Infrared Fluorescent Probe Specific for Hydrogen Peroxide in Living Systems.基于α-酮酰胺的近红外荧光探针用于活细胞中过氧化氢的特异性检测：合理设计。
Anal Chem. 2016 Aug 16;88(16):8019-25. doi: 10.1021/acs.analchem.6b01256. Epub 2016 Aug 4.