School of Electrical, Computer and Energy Engineering at Arizona State University, Suite 334, Goldwater Center, Tempe, AZ 85287, USA.
School of Electrical, Computer and Energy Engineering at Arizona State University, Suite 334, Goldwater Center, Tempe, AZ 85287, USA; Center for Personalized Diagnostics, Biodesign Institute at Arizona State University, Tempe, AZ 85281, USA.
Biosens Bioelectron. 2018 Oct 15;117:153-160. doi: 10.1016/j.bios.2018.04.002. Epub 2018 Apr 4.
An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3 × 3 mm interference filters with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters, we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of ∼1×10photons/mmsec and has the potential for 2-3 orders of magnitude improvement in sensitivity over standard colorimetric detection that uses colored latex microspheres. We also detail the design, development, and characterization of our low-cost fluorescence detection platform and demonstrate 100% and 97.96% reduction in crosstalk probability and filter cost, respectively. This is achieved by reducing filter dimensions and ensuring appropriate channel isolation in a 2 × 2 array configuration. Practical considerations with low-cost interference filter system design, analysis, and system performance are also discussed. The performance of our platform is compared to that of a standard laboratory array scanner. We also demonstrate the detection of antibodies to human papillomavirus (HPV16) E7 protein, as a potential biomarker for early cervical cancer detection in human plasma.
一种有效的传染病防治方法是在护理点 (POC) 部署手持式设备，用于筛查或自我监测应用。需要非常敏感、低成本和定量的诊断设备。在本研究中，我们提出了一种低成本、多路复用荧光检测平台，具有高灵敏度和宽动态范围。我们的系统采用了具有高截止带抑制比、陡峭的过渡边缘和大于 90%的通带传输的廉价 3×3mm 干涉滤光片。除了滤光片之外，我们还通过利用电荷积分读取来提高准确性的时间来提高信噪比。荧光传感平台对光子通量的灵敏度约为 1×10 个光子/mmsec，并且具有比使用彩色乳胶微球的标准比色检测提高 2-3 个数量级灵敏度的潜力。我们还详细介绍了我们低成本荧光检测平台的设计、开发和特性，并分别展示了 100%和 97.96%的串扰概率和滤光片成本降低。这是通过减小滤光片尺寸并在 2×2 阵列配置中确保适当的通道隔离来实现的。还讨论了低成本干涉滤光片系统设计、分析和系统性能的实际考虑因素。我们平台的性能与标准实验室阵列扫描仪进行了比较。我们还展示了人乳头瘤病毒 (HPV16) E7 蛋白抗体的检测，作为人血浆中早期宫颈癌检测的潜在生物标志物。
