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采用新的生物测定系统评估热和新鲜人血清对III型人嗜T细胞病毒感染性的影响。

Effect of heat and fresh human serum on the infectivity of human T-cell lymphotropic virus type III evaluated with new bioassay systems.

作者信息

Harada S, Yoshiyama H, Yamamoto N

出版信息

J Clin Microbiol. 1985 Dec;22(6):908-11. doi: 10.1128/jcm.22.6.908-911.1985.

DOI:10.1128/jcm.22.6.908-911.1985
PMID:2999187
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271849/
Abstract

MT-4 cells, which are a human T-cell lymphotropic virus type I (HTLV-I)-positive cell line highly permissive to HTLV-III infection, were used to detect the biologically active virus. For quantitation of the virus, induction of HTLV-III-specific antigen(s) and inhibition of DNA synthesis in infected MT-4 cells were assessed by indirect immunofluorescence and by a proliferation assay measuring [3H]thymidine uptake, respectively. HTLV-III was fully inactivated by treatment at 56 degrees C for 30 min. It was not inactivated by treatment with fresh anti-HTLV-III-negative serum. Thus, these assay systems with MT-4 cells would be useful in further studies on acquired immune deficiency syndrome.

摘要

MT - 4细胞是一种对人类嗜T细胞病毒I型（HTLV - I）呈阳性的细胞系，对HTLV - III感染高度敏感，被用于检测具有生物活性的病毒。为了对病毒进行定量分析，分别通过间接免疫荧光法评估感染的MT - 4细胞中HTLV - III特异性抗原的诱导情况，并通过测量[3H]胸腺嘧啶核苷摄取的增殖试验来评估DNA合成的抑制情况。HTLV - III在56摄氏度下处理30分钟后完全失活。用新鲜的抗HTLV - III阴性血清处理后它并未失活。因此，这些使用MT - 4细胞的检测系统将有助于获得性免疫缺陷综合征的进一步研究。

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Transformation of human leukocytes by cocultivation with an adult T cell leukemia virus producer cell line.通过与成人T细胞白血病病毒产生细胞系共培养实现人白细胞的转化。
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