Strelkauskas A J, Wilson B S, Callery R T, Chess L, Schlossman S F
J Exp Med. 1977 Dec 1;146(6):1765-72. doi: 10.1084/jem.146.6.1765.
We have investigated the influence of human T cells on the synthesis and secretion of immunoglobulin by peripheral blood B cells. The plaque-forming assay used, which identified the number of B cells secreting Ig, is a short-term assay which requires no exogenous stimulation. We have shown that the B-cell population alone contains fewer secreting cells than the total lymphocyte population, and that T cells are required to achieve maximal plaque-forming cell levels. Cycloheximide treatment of cells at concentrations known to inhibit protein synthesis does not affect the cooperative potential of these cells. Additionally, this cooperation effect is markedly better among autologous mixtures of Ig- and Ig+ cells, than among mixtures obtained from randomly selected individuals.
我们研究了人T细胞对外周血B细胞免疫球蛋白合成与分泌的影响。所采用的空斑形成试验可鉴定分泌Ig的B细胞数量，这是一种短期试验，无需外源刺激。我们已经表明，仅B细胞群体中分泌细胞的数量少于总淋巴细胞群体，并且需要T细胞才能达到最大空斑形成细胞水平。用已知抑制蛋白质合成的浓度的环己酰亚胺处理细胞，并不影响这些细胞的协同潜力。此外，Ig - 和Ig + 细胞的自体混合物之间的这种协同效应明显优于从随机选择的个体获得的混合物之间的协同效应。
