Chi Jun, Li Miaomiao, Lian Feihe, Li Yixiao, Dai Liping
Henan Collaborative Innovation Center for Research and Development on the Whole Industry Chain of Yu-Yao, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Molecules. 2025 May 28;30(11):2349. doi: 10.3390/molecules30112349.
tubers, abundantly produced in southwestern China, are commonly used as a folk medicine that excel in anti-inflammation to treat enteritis, bronchitis, and tonsillitis. In this study, three previously undescribed cucurbitane glycosides, hemchinins G-H (-), that were characterized by the presence of four glucose substitutions, as well as eleven ones with one to three -glucoses, were isolated from the tubers of . The structures were confirmed using comprehensive UV, IR, HR-ESI-MS, and NMR analyses, and absolute configurations were determined through a comparison of calculated and experimental ECD after acid hydrolysis. Compounds - showed NO inhibition effects on LPS-induced RAW 264.7 cells. Finally, molecular docking analyses were conducted to obtain the affinities of the isolated cucurbitane glycosides and our previously reported 19 cucurbitane triterpenes, focusing on targets involved in anti-inflammatory effects. The results indicated that they showed high docking scores of affinities with the proteins in the NF-κB, AMPK, and Nrf2 signaling pathways. Among them, cucurbitane triterpenes with sugar moiety substitution at C-3 and C-26/27 showed better affinity ability. The findings can provide insights into the anti-inflammatory mechanisms of this species and facilitate the development of novel therapeutic agents.
块茎在中国西南部大量产出，通常被用作一种民间药物，在抗炎方面表现出色，可用于治疗肠炎、支气管炎和扁桃体炎。在本研究中，从[植物名称]的块茎中分离出三种此前未被描述的葫芦烷糖苷，即具有四个葡萄糖取代基的hemchinins G-H (-)，以及十一种带有一至三个葡萄糖的糖苷。通过全面的紫外、红外、高分辨电喷雾电离质谱和核磁共振分析确定了其结构，并在酸水解后通过比较计算和实验的电子圆二色光谱确定了绝对构型。化合物[具体化合物编号]对脂多糖诱导的RAW 264.7细胞没有抑制作用。最后，进行了分子对接分析，以获得分离出的葫芦烷糖苷与我们之前报道的19种葫芦烷三萜的亲和力，重点关注参与抗炎作用的靶点。结果表明，它们与核因子κB、腺苷酸活化蛋白激酶和核因子E2相关因子2信号通路中的蛋白质具有较高的对接亲和力得分。其中，在C-3和C-26/27处有糖基部分取代的葫芦烷三萜表现出更好的亲和能力。这些发现可以为该物种的抗炎机制提供见解，并促进新型治疗药物的开发。
