Balabanova H, Kotler M, Becker Y
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2794-8. doi: 10.1073/pnas.72.7.2794.
A fibroblast-like cell culture was established from a stomach biopsy of a patient with metastatic adenocarcinoma. One of the cultures, at the 6th passage level, left unattended for a month at 37 degrees, produced numerous foci of epithelioid cells. Upon subculturing, an epithelioid cell line, designated HCCL (human carcinoma cell line), was established. The HCCL cells released particles possessing the characteristics of oncornaviruses: density 1.175 g/ml, cores with a density of 1.22-1.26 g/ml, high-molecular-weight RNA (60-70S) and RNA-instructed DNA polymerase activity (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). Inoculation of particles released from HCCL cells into cultures of human embryo muscle fibroblasts resulted in the appearance of foci of transformed cells.
从一名转移性腺癌患者的胃活检组织中建立了成纤维细胞样细胞培养物。其中一种培养物在第6代时，在37℃下无人照管一个月后，产生了许多上皮样细胞灶。传代培养后，建立了一种上皮样细胞系，命名为HCCL（人癌细胞系）。HCCL细胞释放出具有肿瘤病毒特征的颗粒：密度为1.175 g/ml，核心密度为1.22 - 1.26 g/ml，高分子量RNA（60 - 70S）以及RNA指导的DNA聚合酶活性（脱氧核苷三磷酸：DNA脱氧核苷酸转移酶，EC 2.7.7.7）。将HCCL细胞释放的颗粒接种到人胚胎肌肉成纤维细胞培养物中，导致出现转化细胞灶。
