Horwitz D A, Lobo P I
J Clin Invest. 1975 Dec;56(6):1464-72. doi: 10.1172/JCI108227.
Two separate lymphocyte populations, each bearing easily detectable surface immunoglobulin, have been detected in human peripheral blood. The first, B cells, has surface determinants that are stable at 37degreeC, but are removed by pronase and regenerate in culture. The cells are nylon adherent and have a receptor for C3, and studies with unit gravity sedimentation indicate they are mostly small lymphocytes. B cells comprise 9.5% of the total lymphocytes, with the normal range from 3-16%. As many or more lymphocytes lack membrane-incorporated Ig determinants but have an Fc receptor that binds IgG1 and IgG3 in normal serum maximally at 4degreeC. This receptor for cytophilic IgG is removed by pronase but not by trypsin. The second population has been named L lymphocytes because of membrane-labile IgG determinants. L cells do not adhere to nylon, do not form rosettes with sheep erythrocytes sensitized with antibody and mouse complement, and are larger than small lymphocytes. These lymphocytes with cold-reactive Fc receptors for serum IgG do not form E-rosettes or respond to phytohemaggutinin. Since L cells do not have surface markers of T and B lymphocytes, it is likely that they comprise a separate population.
在人外周血中已检测到两个不同的淋巴细胞群体，每个群体都带有易于检测的表面免疫球蛋白。第一个群体是B细胞，其表面决定簇在37℃时稳定，但可被链霉蛋白酶去除并在培养中再生。这些细胞能黏附于尼龙纤维，具有C3受体，单位重力沉降研究表明它们大多是小淋巴细胞。B细胞占淋巴细胞总数的9.5%，正常范围为3%至16%。许多或更多的淋巴细胞缺乏膜结合的Ig决定簇，但具有Fc受体，该受体在4℃时能最大程度地结合正常血清中的IgG1和IgG3。这种嗜细胞性IgG的受体可被链霉蛋白酶去除，但不能被胰蛋白酶去除。第二个群体因膜不稳定的Ig决定簇而被命名为L淋巴细胞。L细胞不黏附于尼龙纤维，不与用抗体和小鼠补体致敏的绵羊红细胞形成花环，且比小淋巴细胞大。这些具有对血清IgG的冷反应性Fc受体的淋巴细胞不形成E花环，也不响应植物血凝素。由于L细胞不具有T和B淋巴细胞的表面标志物，它们很可能构成一个独立的群体。
