乙肝抗原相关的脱氧核糖核酸聚合酶活性及e抗原/e抗体系统
Hepatitis B antigen-associated deoxyribonucleic acid polymerase activity and e antigen/anti-e system.
作者信息
Imai M, Tachibana F C, Moritsugu Y, Miyakawa Y, Mayumi M
出版信息
Infect Immun. 1976 Sep;14(3):631-5. doi: 10.1128/iai.14.3.631-635.1976.
Abstract
Serum samples of 403 asymptomatic blood donors carrying hepatitis B surface antigen (HB5Ag) were concentrated threefold and tested for e antigen and antibody to e antigen (anti-e) by immunodiffusion. Hepatitis B antigen (HBAg)-associated deoxyribonucleic acid (DNA) polymerase activity was specifically determined by the difference in incorporation of [methyl-3H]thymidine 5' -triphosphate into DNA by an aliquot of centrifuged serum samples after it had been treated either with normal rabbit serum or with rabbit antibody to HBSAg. All of 58 serum samples containing e antigen revealed HBAg-associated DNA polymerase activity, whereas none of 96 samples containing anti-e did. In the remaining 249 samples in which neither e antigen nor anti-e was found, 62 showed specific DNA polymerase activity, although at lower levels than the samples containing e antigen.
摘要
对403名携带乙型肝炎表面抗原（HBsAg）的无症状献血者的血清样本进行了三倍浓缩，并通过免疫扩散法检测e抗原和e抗原抗体（抗-e）。通过比较经正常兔血清或兔抗-HBsAg抗体处理后的离心血清样本等分试样中[甲基-3H]胸苷5'-三磷酸掺入DNA的差异，特异性测定乙型肝炎抗原（HBAg）相关的脱氧核糖核酸（DNA）聚合酶活性。所有58份含e抗原的血清样本均显示出HBAg相关的DNA聚合酶活性，而96份含抗-e的样本均未显示。在其余249份未检测到e抗原和抗-e的样本中，62份显示出特异性DNA聚合酶活性，尽管其水平低于含e抗原的样本。
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