Diverse Secondary Metabolites from the Coral-Derived Fungus SCSIO 5Bn003.

Three new metabolites, including a cyclic tetrapeptide asperhiratide (), an ecdysteroid derivative asperhiratine (), and a sesquiterpene lactone asperhiratone (), were isolated and identified from the soft coral-derived fungus SCSIO 5Bn003, together with 10 known compounds. Their structures were elucidated via spectroscopic analysis, X-ray diffraction analysis, and electronic circular dichroism calculations. In addition, the absolute configuration of was determined by Marfey's technique and an analysis of the acid hydrolysates using a chiral phase HPLC column. Among all the compounds, and showed medium cytotoxic activities against four tumor cell lines (SF-268, HepG-2, MCF-7, and A549), with IC values ranging from 31.03 ± 3.04 to 50.25 ± 0.54 µM. Meanwhile, they strongly inhibited α-glucosidase activities, with IC values of 35.73 ± 3.94 and 22.00 ± 2.45 µM, which were close to and even stronger than the positive control acarbose (IC = 32.92 ± 1.03 µM). Compounds - showed significant antibacterial activities against , with MIC values of 10.26 ± 0.76 µM, 17.00 ± 1.25 µM, and 5.30 ± 0.29 µM, respectively. Compounds and exhibited potent radical scavenging activities against DPPH, with IC values of 12.23 ± 0.78 µM and 7.38 ± 1.16 µM. In addition, asperhiratide () was evaluated for anti-angiogenic activities in the in vivo zebrafish model, which showed a weak inhibitory effect on intersegmental vessel (ISV) formation.