Van Horn K G, Vandernoot A M, Burke E W, McKitrick J C
J Clin Microbiol. 1984 Oct;20(4):630-5. doi: 10.1128/jcm.20.4.630-635.1984.
The MICs of 10 antimicrobial agents were determined for 1,000 clinical aerobic and facultatively anaerobic gram-negative bacilli by the 6-h AutoMicrobic system Gram-Negative General Susceptibility-Plus Card (GSC+) (Vitek Systems, Inc., Hazelwood, Mo.) and by an 18-h reference agar dilution method. Results obtained by both systems were evaluated for twofold dilution discrepancies and for interpretive category discrepancies. MICs that differed by more than one twofold dilution between the two test systems were considered to be discrepant. The overall twofold dilution agreement of the GSC+ MICs to agar dilution MICs was 94.0%. The agreement for each drug tested ranged from 91.7% (ampicillin and chloramphenicol) to 96.5% (carbenicillin). Interpretive categories were considered in agreement when the susceptibility interpretations obtained by the two systems were both very susceptible, moderately susceptible, or resistant. The overall interpretive category agreement was 80.5%. This agreement increased to 95.8% when all minor errors (moderately susceptible, one system; very susceptible, the other system) that would not affect therapy were omitted. The AutoMicrobic system GSC+ provides rapid and accurate susceptibility test results for clinical aerobic and facultatively anaerobic gram-negative bacilli.
采用6小时自动微生物系统革兰氏阴性通用药敏增强卡片（GSC +）（Vitek系统公司，密苏里州黑兹尔伍德）和18小时参考琼脂稀释法，对1000株临床需氧和兼性厌氧革兰氏阴性杆菌测定了10种抗菌药物的最低抑菌浓度（MIC）。对两种系统获得的结果进行了两倍稀释差异和解释类别差异的评估。两种测试系统之间相差超过一个两倍稀释倍数的MIC被认为是有差异的。GSC +的MIC与琼脂稀释法MIC的总体两倍稀释一致性为94.0%。每种测试药物的一致性范围为91.7%（氨苄西林和氯霉素）至96.5%（羧苄西林）。当两种系统获得的药敏解释均为极敏感、中度敏感或耐药时，认为解释类别一致。总体解释类别一致性为80.5%。当所有不影响治疗的微小误差（一个系统为中度敏感，另一个系统为极敏感）被忽略时，该一致性提高到95.8%。自动微生物系统GSC +为临床需氧和兼性厌氧革兰氏阴性杆菌提供快速准确的药敏试验结果。
