Larese Filon Francesca, Maina Giovanni, Adami Gianpiero, Venier Marta, Coceani Nicoletta, Bussani Rossana, Massiccio Marilena, Barbieri Pierluigi, Spinelli Paolo
UCO Medicina del Lavoro, Università di Trieste, Via della Pietà 19, 34129 Trieste, Italy.
Int Arch Occup Environ Health. 2004 Feb;77(2):85-9. doi: 10.1007/s00420-003-0455-4. Epub 2003 Jun 19.
To evaluate skin absorption of cobalt powder in an in vitro system. Experiments with volunteers show that cobalt powder can permeate through the skin, but there are no data with regard to the mechanism or the amount of permeation.
Skin permeation was calculated by the Franz diffusion cell method with human skin. A physiological solution was used as receiving phase and the cobalt powder was dispersed in synthetic sweat. The amount of metal passing through the skin was analysed by electro-thermal atomic absorption spectrometry (ETAAS). Parallel polarographic analysis (differential pulse polarography-DPP) allowed evaluation of cobalt present as ions (Co(2+)) in donor and receiving phases. Measurements of cobalt skin content were also performed.
Evaluation of metal in the receiving phase allowed us to demonstrate the permeation of cobalt through the skin. Steady-state flow of percutaneous cobalt permeation was calculated as 0.0123+/-0.0054 microg cm(-2) h(-1), with a lag time of 1.55+/-0.71 h.
The experiments show that cobalt powder can pass through the skin when applied as a dispersion in synthetic sweat, oxidising metallic cobalt into ions, which permeate the skin. These experiments show for the first time how cobalt can permeate the skin.
在体外系统中评估钴粉的皮肤吸收情况。志愿者实验表明钴粉可透过皮肤，但关于渗透机制或渗透量尚无数据。
采用Franz扩散池法和人体皮肤计算皮肤渗透率。以生理溶液作为接收相，将钴粉分散于合成汗液中。通过电热原子吸收光谱法（ETAAS）分析透过皮肤的金属量。平行极谱分析（差分脉冲极谱法 - DPP）可评估供体相和接收相中以离子形式（Co(2+)）存在的钴。还进行了钴皮肤含量的测量。
对接收相中金属的评估使我们能够证明钴可透过皮肤。经皮钴渗透的稳态流量计算为0.0123±0.0054微克·厘米(-2)·小时(-1)，滞后时间为1.55±0.71小时。
实验表明，当钴粉以分散于合成汗液中的形式应用时，可透过皮肤，将金属钴氧化为离子，进而渗透皮肤。这些实验首次展示了钴渗透皮肤的方式。
